Topics covered by the training school
Training school on germinal cells and assisted reproductive technologies in farm animals
The second CryoStore training school will take place in Rennes, France, from 2nd to 9th July 2025. Here are more details on the topics that will be covered during the school.
Version 1 - 31.03.2025Lectures on the biology of germinal cells, farm animal reproduction and assisted reproductive biotechnologies
Biology of the germinal stem cells in mammals and in fish, actors of their renewal and differentiation fates
Dr Jean-Jacques Lareyre, INRAE Rennes, France
Biotechnologies using germinal stem cells for surrogate gamete production in fish species: from the first proofs of concept to practical application
Dr Martin Psenicka, Faculty of Fisheries and Protection of Waters - University of South Bohemia Vodnany, Czech Republic
Identification of key factors to improve pluripotency and chimeric gamete production in rabbit
Dr Nathalie Beaujean, INRAE Institut cellules souches et cerveau Lyon, France
Gametes biology and maternal specificities during in vivo fertilization in bovine - part I
Application to in vitro fertilization – part II
Prof. Marie Saint Dizier, INRAE Nouzilly, France
The role of extracellular vesicles in reproductive tract functions
Dr Pierre Comizzoli, National Zoo & Conservation Biology Institute, Smithsonian Organization, USA
“Re-introduction” to cryobiology applied to cryopreservation of germinal cells and tissues
Dr Catherine Labbé, INRAE Rennes, France
The transfer of cryopreservation research to the industry: the view from IMV Technologies by a former PhD student
Dr Lucie Gavin-Plagne, IMV Technologies L’Aigle, France
The biotechnology of sperm and embryo sexing and the biological constraints
Dr Rosa Lino Neto Pereira, INIAV, Portugal
Embryo transfer in swine: biological and practical knowledge
Dr Julie Lamy, Wageningen University and Research (WUR), The Netherlands
Animal Welfare
Animal welfare – part I: Animal welfare: ethics, pain perception, legislation and the 3Rs
Animal welfare – part II: The welfare of production animals, from fish to mammals
Prof. Ian Mayer, NMBU Aas, Norway
Practical courses
All practical courses on embryos will be introduced by a presentation of the animal welfare issues. All doctoral candidates will practice the methods listed below (except for the demo on vitrification).
Germinal stem cells isolation from immature testis in rainbow trout. Grafting into recipient fries at hatching stage by micromanipulation under a stereomicroscope. Demo of the vitrification of testis fragments and ultra-fast thawing (4h)
Introduction to the principle of flow cytometry, finding, counting and assessing testicular cell quality (2 h)
Primordial germ cell (PGCs) collection from hen embryo artery, observation of cultured PGCs, microinjection of sham PGCs into hen surrogate embryos (3 h)
The CASA system (computer-assisted semen analysis) to measure sperm motility in different species: the adaptations required according to the species, the risks of misinterpretation, the power of the method (2h)
Technical visit of the Synetics cattle cooperative and CryoAqua cryobank
Observation of bull semen collection and sperm processing
Presentation of the functioning of SYNETICS in the cattle genetic resources industry
Stéphane Basquin, Synetics Saint Aubin du Cormier, France
Visit of the French cryobank for aquatic species
Presentation of the uses and needs for the selective breeding industry in aquatic species
Pierrick Haffray, SYSAAF Rennes, France
Transferable skills training
Improve outreach activity to the general public: how to identify a compelling story from your research, Stop-Motion animations, videos interviews, social media post, and emerging technologies (AI) (~9h)
Hasan Abbas, GRID Arendal, Norway
Practical scientific writing: how to get started with your manuscript (3,5 h)
Dr Denis Poinsot, OSUR Rennes, France
Gender mainstream workshop
Professional inequalities related to gender stereotypes
Dr Louise Van-Oudenhove, Institut Sophia Agrobiotech, Lyon, France
Privileges and biased visions of other people in research: general discussion from short video examples
All attendants (students and supervisors)
